Alain Charbit - PoSI-Lab

Alain Charbit, PhD, is Emeritus Director of Research (DREM) at CNRS since 2022. He was recruited CR1 by the CNRS in 1991. After 15 years at the Institut Pasteur (Paris), he joined in 1998 the « Faculté de Médecine Paris Descartes » where he is currently co-heading team 7 at the Institut Necker-Enfants Malades.
Alain Charbit is former Director of the Structure Fédérative de recherche (SFR) Necker (Inserm US24, CNRS UMR3633) https://www.necker.fr//sfr-necker/

Last News of the Lab

Airway environment promote Staphylococcus aureus chronic lifestyle

11/12/2023Airway environment drives the selection of quorum sensing mutants and promote Staphylococcus aureus chronic lifestyleXiongqi Ding et al. Nat Commun 2023 Staphylococcus ...

Congratulation to Morgane Wuckelt and Elif UYAR

24/07/2023 .Morgane Wuckelt and Elif Uyar have been awarded a PhD Track Grant from BioSPC. We are all very proud ...

Genomic analysis of S. aureus sequential isolates

27/03/2023 . Genomic analysis of Staphylococcus aureus sequential isolates from lungs of patients with cystic fibrosisDing et al Microbes and ...

Lung-Adapted Staphylococcus aureus Trigger a Proinflammatory Response

07/05/2022 . Lung-Adapted Staphylococcus aureus Isolates With Dysfunctional Agr System Trigger a Proinflammatory ResponseElodie Ramond et al The Journal of ...

The pentose phosphate pathway in pathogenic Francisella

03/08/2021 .The pentose phosphate pathway constitutes a major metabolic hub in pathogenic FrancisellaHéloise Rytter et al PLOS Pathogens 2021. Metabolic ...

Congratulation to Audrey LAURENT, Clémence MOUVILLE and Xiali FU

02/08/2021 .Audrey LAURENT and Clémence MOUVILLE have been awarded a PhD Track Grant from BioSPC. Xiali FU has been awarded ...

MRSA Virulence in a new Drosophila Larval Model

23/06/2021 .Reactive Oxygen Species-Dependent Innate Immune Mechanisms Control Methicillin-Resistant Staphylococcus aureus Virulence in the Drosophila Larval ModelElodie Ramond et al ...

High resolution typing of Staphylococcus epidermidis

28/12/2020 .High resolution typing of Staphylococcus epidermidis based on cgMLST to investigate the hospital spread of multidrug resistant clones. Jamet et al ...

A Sheath Phosphorylation control T6SS Assembly

Critical Role of a Sheath Phosphorylation Site On the Assembly and Function of an Atypical Type VI Secretion System. Ziveri ...

Chronic Staphylococcus Aureus Lung Infection Correlates With an Increased Intracellular Persistence

Chronic Staphylococcus Aureus Lung Infection Correlates With Proteogenomic and Metabolic Adaptations Leading to an Increased Intracellular Persistence. Tan et al, ...

Team Members

Last 25 Publications

Rytter, H, Roger, K, Chhuon, C, Ding, X, Coureuil, M, Jamet, A et al.. Dual proteomics of infected macrophages reveal bacterial and host players involved in the Francisella intracellular life cycle and cell to cell dissemination by merocytophagy. Sci Rep. 2024;14 (1):7797. doi: 10.1038/s41598-024-58261-x. PubMed PMID:38565565 PubMed Central PMC10987565.
Ding, X, Robbe-Masselot, C, Fu, X, Léonard, R, Marsac, B, Dauriat, CJG et al.. Airway environment drives the selection of quorum sensing mutants and promote Staphylococcus aureus chronic lifestyle. Nat Commun. 2023;14 (1):8135. doi: 10.1038/s41467-023-43863-2. PubMed PMID:38065959 PubMed Central PMC10709412.
Ding, X, Fu, X, Euphrasie, D, Ferroni, A, Sermet-Gaudelus, I, Charbit, A et al.. Genomic analysis of Staphylococcus aureus sequential isolates from lungs of patients with cystic fibrosis. Microbes Infect. 2023;25 (6):105124. doi: 10.1016/j.micinf.2023.105124. PubMed PMID:36871931 .
Ramond, E, Lepissier, A, Ding, X, Bouvier, C, Tan, X, Euphrasie, D et al.. Lung-Adapted Staphylococcus aureus Isolates With Dysfunctional Agr System Trigger a Proinflammatory Response. J Infect Dis. 2022;226 (7):1276-1285. doi: 10.1093/infdis/jiac191. PubMed PMID:35524969 .
Jung, V, Roger, K, Chhuon, C, Pannetier, L, Lipecka, J, Gomez, JS et al.. BLI-MS: Combining biolayer interferometry and mass spectrometry. Proteomics. 2022;22 (9):e2100031. doi: 10.1002/pmic.202100031. PubMed PMID:34958708 .
Rytter, H, Jamet, A, Ziveri, J, Ramond, E, Coureuil, M, Lagouge-Roussey, P et al.. The pentose phosphate pathway constitutes a major metabolic hub in pathogenic Francisella. PLoS Pathog. 2021;17 (8):e1009326. doi: 10.1371/journal.ppat.1009326. PubMed PMID:34339477 PubMed Central PMC8360588.
Ramond, E, Jamet, A, Ding, X, Euphrasie, D, Bouvier, C, Lallemant, L et al.. Reactive Oxygen Species-Dependent Innate Immune Mechanisms Control Methicillin-Resistant Staphylococcus aureus Virulence in the Drosophila Larval Model. mBio. 2021;12 (3):e0027621. doi: 10.1128/mBio.00276-21. PubMed PMID:34126772 PubMed Central PMC8262968.
Rytter, H, Jamet, A, Coureuil, M, Charbit, A, Ramond, E. Which Current and Novel Diagnostic Avenues for Bacterial Respiratory Diseases?. Front Microbiol. 2020;11 :616971. doi: 10.3389/fmicb.2020.616971. PubMed PMID:33362754 PubMed Central PMC7758241.
Jamet, A, Guglielmini, J, Brancotte, B, Coureuil, M, Euphrasie, D, Meyer, J et al.. High-Resolution Typing of Staphylococcus epidermidis Based on Core Genome Multilocus Sequence Typing To Investigate the Hospital Spread of Multidrug-Resistant Clones. J Clin Microbiol. 2021;59 (3):. doi: 10.1128/JCM.02454-20. PubMed PMID:33328176 PubMed Central PMC8106705.
Quereda, JJ, Morel, C, Lopez-Montero, N, Ziveri, J, Rolland, S, Grenier, T et al.. A Role for Taok2 in Listeria monocytogenes Vacuolar Escape. J Infect Dis. 2022;225 (6):1005-1010. doi: 10.1093/infdis/jiaa367. PubMed PMID:32582947 PubMed Central PMC8922001.
Tan, X, Coureuil, M, Charbit, A, Jamet, A. Multitasking Actors of Staphylococcus aureus Metabolism and Virulence. Trends Microbiol. 2020;28 (1):6-9. doi: 10.1016/j.tim.2019.10.013. PubMed PMID:31753539 .
Ramond, E, Jamet, A, Coureuil, M, Charbit, A. Pivotal Role of Mitochondria in Macrophage Response to Bacterial Pathogens. Front Immunol. 2019;10 :2461. doi: 10.3389/fimmu.2019.02461. PubMed PMID:31708919 PubMed Central PMC6819784.
Ziveri, J, Chhuon, C, Jamet, A, Rytter, H, Prigent, G, Tros, F et al.. Critical Role of a Sheath Phosphorylation Site On the Assembly and Function of an Atypical Type VI Secretion System. Mol Cell Proteomics. 2019;18 (12):2418-2432. doi: 10.1074/mcp.RA119.001532. PubMed PMID:31578219 PubMed Central PMC6885697.
Tan, X, Ramond, E, Jamet, A, Barnier, JP, Decaux-Tramoni, B, Dupuis, M et al.. Transketolase of Staphylococcus aureus in the Control of Master Regulators of Stress Response During Infection. J Infect Dis. 2019;220 (12):1967-1976. doi: 10.1093/infdis/jiz404. PubMed PMID:31420648 .
Vidal, C, Moulin, F, Nassif, X, Galmiche, L, Borgel, D, Charbit, A et al.. Fulminant arterial vasculitis as an unusual complication of disseminated staphylococcal disease due to the emerging CC1 methicillin-susceptible Staphylococcus aureus clone: a case report. BMC Infect Dis. 2019;19 (1):302. doi: 10.1186/s12879-019-3933-3. PubMed PMID:30943907 PubMed Central PMC6446405.
Tan, X, Coureuil, M, Ramond, E, Euphrasie, D, Dupuis, M, Tros, F et al.. Chronic Staphylococcus aureus Lung Infection Correlates With Proteogenomic and Metabolic Adaptations Leading to an Increased Intracellular Persistence. Clin Infect Dis. 2019;69 (11):1937-1945. doi: 10.1093/cid/ciz106. PubMed PMID:30753350 .
Le Gallou, S, Zhou, Z, Thai, LH, Fritzen, R, de Los Aires, AV, Mégret, J et al.. A splenic IgM memory subset with antibacterial specificities is sustained from persistent mucosal responses. J Exp Med. 2018;215 (8):2035-2053. doi: 10.1084/jem.20180977. PubMed PMID:29959173 PubMed Central PMC6080908.
Brauge, T, Faille, C, Sadovskaya, I, Charbit, A, Benezech, T, Shen, Y et al.. The absence of N-acetylglucosamine in wall teichoic acids of Listeria monocytogenes modifies biofilm architecture and tolerance to rinsing and cleaning procedures. PLoS One. 2018;13 (1):e0190879. doi: 10.1371/journal.pone.0190879. PubMed PMID:29320565 PubMed Central PMC5761963.
Jamet, A, Charbit, A, Nassif, X. Antibacterial Toxins: Gram-Positive Bacteria Strike Back!. Trends Microbiol. 2018;26 (2):89-91. doi: 10.1016/j.tim.2017.11.003. PubMed PMID:29174101 .
Ziveri, J, Tros, F, Guerrera, IC, Chhuon, C, Audry, M, Dupuis, M et al.. The metabolic enzyme fructose-1,6-bisphosphate aldolase acts as a transcriptional regulator in pathogenic Francisella. Nat Commun. 2017;8 (1):853. doi: 10.1038/s41467-017-00889-7. PubMed PMID:29021545 PubMed Central PMC5636795.
Jamet, A, Touchon, M, Ribeiro-Gonçalves, B, Carriço, JA, Charbit, A, Nassif, X et al.. A widespread family of polymorphic toxins encoded by temperate phages. BMC Biol. 2017;15 (1):75. doi: 10.1186/s12915-017-0415-1. PubMed PMID:28851366 PubMed Central PMC5576092.
Ghenassia, A, Gross, DA, Lorain, S, Tros, F, Urbain, D, Benkhelifa-Ziyyat, S et al.. Intradermal Immunization with rAAV1 Vector Induces Robust Memory CD8⁺ T Cell Responses Independently of Transgene Expression in DCs. Mol Ther. 2017;25 (10):2309-2322. doi: 10.1016/j.ymthe.2017.06.019. PubMed PMID:28720467 PubMed Central PMC5628776.
Barel, M, Charbit, A. Role of Glycosylation/Deglycolysation Processes in Francisella tularensis Pathogenesis. Front Cell Infect Microbiol. 2017;7 :71. doi: 10.3389/fcimb.2017.00071. PubMed PMID:28377902 PubMed Central PMC5359314.
Rigard, M, Bröms, JE, Mosnier, A, Hologne, M, Martin, A, Lindgren, L et al.. Francisella tularensis IglG Belongs to a Novel Family of PAAR-Like T6SS Proteins and Harbors a Unique N-terminal Extension Required for Virulence. PLoS Pathog. 2016;12 (9):e1005821. doi: 10.1371/journal.ppat.1005821. PubMed PMID:27602570 PubMed Central PMC5014421.
Pizarro-Cerdá, J, Charbit, A, Enninga, J, Lafont, F, Cossart, P. Manipulation of host membranes by the bacterial pathogens Listeria, Francisella, Shigella and Yersinia. Semin Cell Dev Biol. 2016;60 :155-167. doi: 10.1016/j.semcdb.2016.07.019. PubMed PMID:27448494 PubMed Central PMC7082150.

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